МОНИТОРИНГ ВИРУСА ЛЕЙКОЗА КРУПНОГО РОГАТОГО СКОТА В КАЗАХСТАНЕ: АНАЛИЗ СЕРОЛОГИЧЕСКИХ И МОЛЕКУЛЯРНЫХ МЕТОДОВ ДИАГНОСТИКИ

Р.К. Нисанова; С.Б. Маманова; А.С. Нурпейсова; С.Е. Каймолдина; П.Б. Акшалова; Е.Э. Башенова; В.В. Кирпиченко; А.С. Карабасова; Д.Т. Тажбаева; А.Ж. Касен; У.Ж. Омарбекова; З.А. Латыпова; В.Ю. Сущих; М.М. Касенов

doi:10.26577/bb202510216

Authors

R.K. Nissanova Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0003-0548-9207 (unauthenticated)
S.B. Mamanova Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0003-2317-8779 (unauthenticated)
A.S. Nurpeisova Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0002-7039-5621 (unauthenticated)
S.E. Kaimoldina Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0002-7658-5805 (unauthenticated)
P.B. Akshalova Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0003-1520-1887 (unauthenticated)
E.E. Bashenova Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0001-6162-2274 (unauthenticated)
V.V. Kirpichenko Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0002-2494-3826 (unauthenticated)
A.S. Karabassova Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0001-6118-0576 (unauthenticated)
D.T. Tazhbayeva Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral, Kazakhstan https://orcid.org/0000-0001-7429-7781 (unauthenticated)
A.Zh. Kasen Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral, Kazakhstan https://orcid.org/0009-0009-9568-0208 (unauthenticated)
U.Zh. Omarbekova Kazakh National Agrarian Research University, Almaty, Kazakhstan https://orcid.org/0000-0002-2459-5438 (unauthenticated)
Z.A. Latypova Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0001-9433-4041 (unauthenticated)
V.Yu. Sushchikh Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0002-3520-2257 (unauthenticated)
M.M. Kassenov Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan https://orcid.org/0000-0001-6124-703X (unauthenticated)

DOI:

https://doi.org/10.26577/bb202510216

Keywords:

bovine leukemia virus, serological methods, RT-PCR, monitoring, Kazakhstan.

Abstract

Bovine leukemia virus (BLV) poses a significant threat to livestock farming in Kazakhstan. This study presents a comprehensive analysis of the prevalence of BLV based on data from 2024, obtained using serological and molecular diagnostic methods. The sensitivity and specificity of agar gel immunodiffusion (AGID), enzyme-linked immunosorbent assay (ELISA), and real-time polymerase chain reaction (RT-PCR) were evaluated. Serological methods demonstrated a high seroprevalence of BLV among animals, with ELISA showing greater sensitivity compared to AGID, confirmed by statistically significant results (p < 0.05). RT-PCR identified fewer active infections, indicating the latent nature of the disease in many infected animals. The correlation coefficient between AGID and RT-PCR was 0.791 (p = 0.011), indicating a high degree of concordance between these methods. However, the correlation between ELISA and RT-PCR was weaker (r = 0.461, p = 0.212), highlighting the difference in the diagnostic nature of these tests. ...

Author Biographies

R.K. Nissanova, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

Lead of virology laboratory LLP «Kazakh Scientific Research Veterinary Institute» (Almaty, Kazakhstan. email: raihan.nisanova@gmail.com)
S.B. Mamanova, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

Senior Research Scientist, Virology Laboratory LLP «Kazakh Scientific Research Veterinary Institute» (Almaty, Kazakhstan. email: sal.71@mail.ru)
A.S. Nurpeisova, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

, PhD, Deputy Director of Production, «Kazakh Scientific Research Veterinary Institute» LLP (Almaty, Kazakhstan. email: ainurnurpeisova@mail.ru)
S.E. Kaimoldina, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

junior Researcher, LLP «Kazakh Scientific Research Veterinary Institute», (Almaty, Kazakhstan. email: sayra_kaymoldina@mail.ru)
P.B. Akshalova, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

Candidate of Veterinary Sciences, senior Researcher, LLP «Kazakh Scientific Research Veterinary Institute» (Almaty, Kazakhstan. email: peri.akshalova@gmail.com)
E.E. Bashenova, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

PhD, senior Researcher, LLP «Kazakh Scientific Research Veterinary Institute», 223 Raiymbek Avenue (Almaty, Kazakhstan. email: eralievna86@mail.ru)
V.V. Kirpichenko, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

Candidate of Veterinary Sciences, Senior Research Scientist, LLP «Kazakh Scientific Research Veterinary Institute» (Almaty, Kazakhstan. email: vlad_92reik@mail.ru)
A.S. Karabassova, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

PhD, senior researcher, LLP «Kazakh Scientific Research Veterinary Institute», (Almaty, Kazakhstan. email: aiken.karabasova@mail.ru)
D.T. Tazhbayeva, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral, Kazakhstan

«Zhangir khan West Kazakhstan Agrarian and Technical University», Uralsk, st. Zhangir khan 51, 090009, Kazakhstan (Uralsk, Kazakhstan. email: t_dauriya_t@inbox.ru)
A.Zh. Kasen, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral, Kazakhstan

junior Researcher, LLP «Kazakh Scientific Research Veterinary Institute», (Almaty, Kazakhstan. email: dilek.inwincible@gmail.com)
U.Zh. Omarbekova, Kazakh National Agrarian Research University, Almaty, Kazakhstan

Candidate of Veterinary Sciences, Professor, "Kazakh National Agrarian Research University", Faculty of "Veterinary", Department of "Biological Safety", (Almaty, Kazakhstan. email: urzanoma-58@mail.ru)
Z.A. Latypova, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

Ph.D. in Biological Sciences, Laboratory of Molecular Genetics and Food Safety, LLC "Kazakh Scientific Veterinary Research Institute", (Almaty, Kazakhstan. email: zalinal@list.ru)
V.Yu. Sushchikh, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

PhD Candidate of Veterinary Sciences, head of the bacteriological laboratory, LLC "Kazakh Scientific Veterinary Research Institute", (Almaty, Kazakhstan. email: vladasali@mail.ru)
M.M. Kassenov , Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan

Candidate of Veterinary Sciences, general manager, «Kazakh Scientific Research Veterinary Institute» LLP (Almaty, Kazakhstan. email: kassenov_mm@mail.ru)