Results of monitoring bulls carrier genetic defects

Abstract

The article provides information about the frequency of occurrence of autosomal recessive lethal mutation – Bovine Leukocyte Adhesion Deficiency  (BLAD), Complex vertebral malformation (CVM), Uridine Monophosphate Synthase  Deficiencies (DUMPS) and Bovine Citrullinaemia  (BC) in breeding bulls  JSC «Asyl-Tulik» (N = 67) and LLP «Asyl» (N = 43) of domestic and foreign selection. It is known that harmful allele mutation frequency is high in cattle Holstein, some local cattle breeds prevalence of these mutations is low or non-existent. Detection of lethal mutations of genes CD 18, SLC35A3, UMP and ASS in bulls (N = 110) was performed by PCR-RFLP analysis and Real-Time PCR SNPs diagnostics. It was found that breeding bulls of local breeds, Alatau, Auliekol and Kazakh white-free carriers of harmful mutations - BLAD, CVM, DUMPS and BC. Two facts are revealed heterozygous carriers of CVM bulls of Holstein and Hereford breeds and one case of heterozygous carriers of BLAD animal Holstein foreign selection. The developed diagnostic method allows for two hours to pinpoint healthy animals homozygous and heterozygous carriers of the mutation. The authors of the first technique used Real-Time PCR SNPs diagnostics to identify the genetic defect DUMPS.