Антиоксидантные и нейропротекторные свойства фитопрепарата из кермека Гмелина
Ключевые слова:
инсульт, астроциты, нейроны, кермек Гмелина, активные формы кислорода.Аннотация
Поиск веществ, подавляющих выработку активных форм кислорода (АФК) и провоспалительных цитокинов в условиях ишемического поражения головного мозга, имеет большое практическое значение для терапии постинсультных состояний. Целью настоящего исследования явилось изучение влияния экстракта из кермека Гмелина на развитие окислительного стресса в астроцитах и нейронах головного мозга человека в условиях воздействия провоспалительного цитокина TNF-α in vitro.В работе использованы методы выделения и культивирования фетальных клеток головного мозга человека, иммуногистохимические, флуоресцентные методы качественного и количественного определения супероксид аниона в выделенных нейронах и астроцитах. В ходе исследования было установлено, что экстракт из кермека Гмелина при дозировке 30 мкг/мл не обладает цитоцидным эффектом, более того, он нивелирует токсичное действие TNF–α: блокирует образование АФК в нейронах и астроцитах, препятствует активации НАДФН оксидазы в астроцитах. Таким образом результаты проведенного исследования позволяют заключить, что экстракт из кермека Гмелина обладает антиоксидантными, астро- и нейропротекторными свойствами и может быть использован для терапии реперфузионного синдрома. Результаты исследования важны для нейробиологии и медицины.
Библиографические ссылки
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2. Cai H, Griendling KK, Harrison DG (2003) The vascular NAD(P)H oxidases as therapeutic targets in cardiovascular diseases. Trends in Pharmacological Sciences, 24 (9): P. 471-478.
3. Chen H, Yoshioka H, Kim GS, Jung JE, Okami N, Sakata H, Maier CM, Narasimhan P, Goeders CE, Chan PH (2011) Oxidative stress in ischemic brain damage: mechanisms of cell death and potential molecular targets for neuroprotection. Antioxid Redox Signal, 14 (8): 1505-1517.
4. Chuang DY, Chan MH, Zong Y, Sheng W, He Y, Jiang JH, Simonyi A, Gu Z, Fritsche KL, Cui J, Lee JC, Folk WR, Lubahn DB, Sun AY, Sun GY (2013) Magnolia polyphenols attenuate oxidative and inflammatory responses in neurons and microglial cells. J Neuroinflammation, 10: 15.
5. Corda S, Laplace C, Vicaut E, Duranteau J (2001) Rapid reactive oxygen species production by mitochondria in endothelial cells exposed to tumor necrosis factor-alpha is mediated by ceramide. Am J Respir Cell Mol Biol, 24 (6): 762-768.
6. Erkebaeva SK, Nurguzhaev ES, Gafurov BG, Tuksanbaeva GU (2014) Prophylaxis of Stroke in Patients with Cerebral Ischemia with Depressive Syndrome. Neuroscience and Behavioral Physiology, 44 (2): 175-179.
7. Feigin VL, Forouzanfar MH, Krishnamurthi R, Mensah GA, Connor M, Bennett DA, Moran AE, Sacco RL, Anderson L, Truelsen T, O'Donnell M, Venketasubramanian N, Barker-Collo S, Lawes CM, Wang W, Shinohara Y, Witt E, Ezzati M, Naghavi M, Murray C (2014) Global and regional burden of stroke during 1990-2010: findings from the Global Burden of Disease Study 2010. Lancet 383 (9913): 245-254.
8. Jana M, Jana A, Pal U, Pahan K (2007) A simplified method for isolating highly purified neurons, oligodendrocytes, astrocytes, and microglia from the same human fetal brain tissue. Neurochem Res, 32 (12): 2015-2022.
9. Li JM, Shah AM (2002) Intracellular localization and preassembly of the NADPH oxidase complex in cultured endothelial cells. J Biol Chem, 277 (22): 19952-19960.
10. Liu X, Wang Z, Wang P, Yu B, Liu Y, Xue Y (2013) Green tea polyphenols alleviate early BBB damage during experimental focal cerebral ischemia through regulating tight junctions and PKCalpha signaling. BMC Complement Altern Med, 13 (1): 187.
11. Matsuo Y, Onodera H, Shiga Y, Shozuhara H, Ninomiya M, Kihara T, Tamatani T, Miyasaka M, Kogure K (1994) Role of cell adhesion molecules in brain injury after transient middle cerebral artery occlusion in the rat. Brain Res, 656 (2): 344-352.
12. Panickar KS, Jang S (2013) Dietary and plant polyphenols exert neuroprotective effects and improve cognitive function in cerebral ischemia. Recent Pat Food Nutr Agric, 5 (2): 128-143.
13. Park L, Anrather J, Zhou P, Frys K, Pitstick R, Younkin S, Carlson GA, Iadecola C (2005) NADPH Oxidase-Derived Reactive Oxygen Species Mediate the Cerebrovascular Dysfunction Induced by the Amyloid -beta- Peptide. J. Neurosci, 25 (7): 1769-1777.
14. Savman K, Heyes MP, Svedin P., Karlsson A (2013) Microglia/macrophage-derived inflammatory mediators galectin-3 and quinolinic acid are elevated in cerebrospinal fluid from newborn infants after birth asphyxia. Transl Stroke Res, 4 (2): 228-235.
15. Sharif A, Prevot V (2012) Isolation and culture of human astrocytes. Methods Mol Biol, 814: 137-151.
16. Sharma VK, Kawnayn G, Sarkar N (2013) Acute ischemic stroke: comparison of low-dose and standard-dose regimes of tissue plasminogen activator. Expert Rev Neurother, 13 (8): 895-902.
17. Simonyi A, Wang Q, Miller RL, Yusof M, Shelat PB, Sun AY, Sun GY (2005) Polyphenols in cerebral ischemia: novel targets for neuroprotection. Mol Neurobiol, 31 (1-3): 135-147.
18. Smith HK, Gavins FN (2012) The potential of stem cell therapy for stroke: is PISCES the sign? FASEB J, 26 (6): 2239-2252.
19. Soltani MH, Pichardo R, Song Z, Sangha N, Camacho F, Satyamoorthy K, Sangueza OP, Setaluri V (2005) Microtubule-associated protein 2, a marker of neuronal differentiation, induces mitotic defects, inhibits growth of melanoma cells, and predicts metastatic potential of cutaneous melanoma. Am J Pathol, 166 (6): 1841-1850.
20. Tarnowski BI, Spinale FG, Nicholson JH (1991) DAPI as a useful stain for nuclear quantitation. Biotech Histochem, 66 (6): 297-302.
21. Yang X, Askarova S, Sheng W, Chen JK, Sun AY, Sun GY, Yao G, Lee JC (2010) Low energy laser light (632.8 nm) suppresses amyloid-beta peptide-induced oxidative and inflammatory responses in astrocytes. Neuroscience, 171 (3): 859-868.
22. Zanetti M, d'Uscio LV, Peterson TE, Katusic ZS, O'Brien T (2005) Analysis of superoxide anion production in tissue. Methods in molecular medicine, 108: 65-72.
23.Zhusupova G.E. (1997) Fitokhimicheskoe issledovanie kornei kermeka solonchakovogo (Gmelina) [Phytochemical study of the roots of the Limonium gmelinii]. Tezisi IV mezhdunarodnoi konferencii po medicinskoi botanike, P. 393.
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Опубликован
2018-01-01
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Раздел
ФИЗИОЛОГИЯ И БИОХИМИЯ ЧЕЛОВЕКА И ЖИВОТНЫХ
